Streamline gene therapy development and other AAV-based applications

With a simple one-step protocol to concentrate AAV particles from media, you can streamline your gene therapy development and other projects that rely on AAV transduction.

• Easy to use, single reagent
• Isolate rAAV directly from media
• No cell lysates or centrifugation
• Save time and cost-effective
• Compatible with all serotypes

Unlike conventional AAV isolation methods which are labor-intensive, multi-step, and require cell lysis, CsCl₂ density gradient ultracentrifugation, and chromatography, SBI’s AAVanced™ Concentration Reagent isolates rAAV particles from media in a single easy step. Simply add AAVanced Concentration Reagent to AAV particle-containing media, incubate overnight at 4°C, and then spin at 1,500g for thirty minutes. Wash the pellet once, spinning again for only three minutes, and then resuspend and aliquot the concentrated virus. Titers of the isolated AAV particles are similar to titers of AAV particles isolated with traditional methods (Figure 1).

Figure 1. AAVanced Concentration Reagent delivers AAV particle preps with similar titers as AAV particles prepared using conventional methods. rAAV was packaged with a PGK-GFP expression shuttle vector. The rAAV particles were isolated from packaging cell media either by the conventional method—cell lysis freeze-thaw with density gradient ultracentrifugation—or with the AAVanced Concentration Reagent. Equal amounts of rAAV were then added to HEK293 cells to test for infectivity based on GFP expression. Representative images of GFP expressing cells 10-days after infection show similar levels of infectivity.

美国SBI代理 System Biosciences,简称SBI，美国加州湾区新成立的生技公司，致力于独特、创新生物技术之开发，以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰（RNAi）研究之相关工具。 System Biosciences (SBI) 致力于开发独特、革新的技术，为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司，提供基于慢病毒 美国SBI代理 ExoQuick and ExoQuick-TC Products Product Size Catalog # ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1 ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1 Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1 ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1 ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1 ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1 Exosome-depleted FBS Media Supplement - USA Certified 50 ml EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement - USA Certified 250 ml EXO-FBS-250A-1 ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1